Western blot analysis was performed on whole cell extracts (20 µg lysate) of HT-29 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), PC-3 (lane 4), Jurkat (lane 5), HCT 116 (lane 6), K562 (lane 7), COLO 205 (lane 8), PC-12 (lane 9) and A549 (lane 10). The blots were probed with Anti-KLF4 Rabbit Polyclonal Antibody (Product # PA1-095, 1:500-1:1500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 54 kDa band corresponding to KLF4 was observed across cell lines tested expect for PC-12. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).